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Research

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Research Call

DAFM Reference

Lead(Collaborating)Institution

DAFM Award

DAFM National Call 2011 11 S 103 UCD(Teagasc) € 770,202

Project Title:

Breeding of wheat in order to enhance yield and disease resistance - mutation of the BRI1 brassinosteroid receptor gene

Project Coordinator:

Dr. Fiona Doohan

Project Abstract

This project will bring together Irish and U K industrial and agency expertise in order to improve the yield and disease resistance of wheat. It will do so by a non-GM-based mutational breeding approach. The target for mutational breeding is the hormone receptor BRASSINOSTEROID I N S E N S f l l V E 1 (BRIl). The uzu phenotype in barley was originally identified as a spontaneous semi-dwarf mutation in the BRIl gene in Japanese germplasm, and it has been widely introgressed into Asian material to improve both lodging, yield and stress resistance. We recently found that the uzu barley, which has a mutation in the BRIl cytoplasmic kinase domain, has enhanced yield and resistance to Fusarium head blight disease, relative to its parent genotype. Following on from this, we want to use a technique known as T I L L I NG (Targeting induced Local Lesions in Genomes) to screen for chemicallyinduced BRIl mutations in cereal germplasm. Thereafter, we will determine the effect of the BRIl mutation on the yield and broad-spectrum field performance of both wheat and barley. Based on this research we will have ensured there is no detrimental trade-off in breeding for brassinosteroid resistance, we will have determined the yield and disease control potential of such an approach, and we will have provided breeders with the basis of material for introgressing such a trait into their breeding lines.

Final Report:

Not available yet.