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Research Call

DAFM Reference

Lead(Collaborating)Institution

DAFM Award

DAFM National Call 2011 11F009 UCC (Teagasc) €490,393

Project Title:

The use of marine derived antibacterial agents to combat the prevalence of Salmonella in pork products

Project Coordinator:

Prof Alan Dobson

Project Abstract

Infections caused by food borne pathogens, such as Salmonella spp. are a major public health problem worldwide and the consumption of pork products containing salmonellae continues to be a major source of food poisoning. There is a clear need to identify novel products to control the threat both to human health and the pig industry in Ireland. Marine sponge-derived Pseudovibrio species were previously identified (in FIRM project FS067) as a novel source of antiSalmonella activities but the isolation and charactorisation of these anti-Salmonella activities has proved difficult using standard approaches. This new project will build upon these results, using an approach combining genomics, molecular microbiology and natural product chemistry, to fully charactorise antiSalmonella compounds from these Pseudovibrio isolates. Draft genome sequences of three selected bioactive Pseudovibrio species will be determined. These genomes will be analysed for the presence of genes involved in tyhe biosynthesis of known families of antibiotics. Newly identified antibiotic biosynthesis gene clusters will then be over-expressed leading to increased production of antibiotics and enabling their purification and full characterisation. In a parallel approach a media/fermentation optimisation strategy will be employed to increase antibiotic production levels in the native hosts. Anti-Salmonella activities will be purified using a bioassay-guided strategy with novel compounds being rapidly identified using mass spectrometry approach. Novel compounds will be fully characterised by NMR. This novel approach will result in the identification of 3-15 compounds with anti-Salmonella activity and an improved means to produce then for commercialisation and field applications.

Final Report:

Not available yet.