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Research

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Research Call

DAFM Reference

Lead(Collaborating)Institution

DAFM Award

DAFM National Call 2011 11F023 UCC (Teagasc) €234,250

Project Title:

Novel prebiotics from plant-derived sugars using bifidobacterial enzymes

Project Coordinator:

Prof Douwe van Sinderen

Project Abstract

Prebiotics such as trans-galacto- galactosidase enzymes of yeast or bacterial origin, and the disaccharide lactose as the feeding material for (ga)lactose oligomerization. Based on the same oligomerization principle we propose to develop novel prebiotic ingredients based on plant-derived soluble sugars as building blocks and the enzymatic capabilities of selected bifidobacterial enzymes. The - - - glucosidases to be used in the current project have been identified from the genome sequence of the probiotic Bifidobacterium breve strain UCC2003, which will be used as a model system by the applicants. Specifically, this pr - - -glucosidases to produce oligosaccharides (OS) from simple sugars from vegetables and cereals as starting materials. These OS will be characterized and successful OSproduction processes will be optimized and scaled up. It is proposed to scientifically validate their prebiotic efficacy initially in in vitro models and subsequently in an in vivo murine model. In particular, the effect of prebiotic ingestion on the composition of the intestine microbiota will be assessed by pyrosequencing and quantitative PCR.

Final Report:

Not available yet.